|FACTOR V (5) LEIDEN|
|Alternate Names for Test:||
|Constituent Tests:||FVL and Prothrombin Gene Mutation are performed together as a multiplex assay|
|Specimen Collection Requirements:|
|Phlebotomist Instructions:||A single tube is adequate for both Factor V Leiden and Prothrombin G20210A testing,
but cannot be shared with other tests.
NOTE: if patient is <16 years of age, then the requesting clinician must discuss
with a haematologist to obtain approval before phlebotomy. Where approved /
recommended, please provide the clinician's name and the clinical indication for
the test in the clinical details section.
1x Mauve (EDTA) tube 4.0mL
|Minimum Sample Volume:||3 mL Whole blood. No separation - whole blood|
|Alternative Blood Tubes:||Blue (Sodium Citrate) tube 2.7mL|
|Additional Information:||Factor V Leiden is part of Thrombophilia screen.
Thrombophilia testing will be performed for the following clinical indications:
Idiopathic venous thrombo-embolism in young patients (<45 years) Recurrent
Recurrent VTE despite adequate therapeutic anticoagulation
VTE in context of family history of unprovoked VTE in a first degree
Thrombosis in unusual sites (e.g. cerebral, mesenteric, portal) VTE
in association with a history of thrombophlebitis
Warfarin-induced skin necrosis
Pregnancy complications such as pre-eclampsia, IUGR, implantation failure,
placental abruption or recurrent miscarriage.*
Thrombophilia screen will not be performed in the following clinical settings:
Prior to prescription of COC in patients with a personal history of VTE†
Prior to prescription of COC in patients with a family history of VTE†
Arterial thrombosis (Lupus testing is indicated in this setting)
* There is no evidence currently to support anticoagulation in women with
pregnancy complications and a positive hereditary thrombophilia. Trials are
ongoing in this area. However, it is recognised that in routine practice
management decisions are currently made in conjunction with thrombophilia testing.
† Current British guidelines recommend avoidance of the COC in this setting
regardless of the thrombophilia results.
PLEASE NOTE: As of 09.01.2012 Activated Protein C Resistance is no longer
performed as part of a thrombophilia screen.
|Pre-Transport Requirements to Reference Lab:||Room temperature
|Transport Requirements to Reference Lab:||Room temperature
|In the Lab:|
|Turnaround Time:||14 days|
|Method:||LightCycler - PCR|
|Stability Time Limit for Add-On Tests:||No add on testing possible|
|Link to Application and Diagnostic Use Website:||https://www.rcpa.edu.au/Library/Practising-Pathology/RCPA-Manual/Items/Pathology-Tests|
|Lab Contact:||Robyn Mardell|
|Department Name:||Molecular Biology|
|Phone:||(04) 918 5314|
Test Information last updated on 11/01/2017
Website last updated on 16/01/2017 08:04