FACTOR V (5) LEIDEN
Department: Molecular Biology
Alternate Names for Test: PROTHROMBIN G20210A
PROTHROMBIN MUTATION
Constituent Tests: FVL and Prothrombin Gene Mutation are performed together as a multiplex assay
Specimen Collection Requirements:
Phlebotomist Instructions: A single tube is adequate for both Factor V Leiden and Prothrombin G20210A testing, 
but cannot be shared with other tests.

NOTE: if patient is <16 years of age, then the requesting clinician must discuss 
with a haematologist to obtain approval before phlebotomy. Where approved / 
recommended, please provide the clinician's name and the clinical indication for 
the test in the clinical details section.
Sample Type:

1x Mauve (EDTA) tube 4.0mL

Minimum Sample Volume: 3 mL Whole blood. No separation - whole blood
Alternative Blood Tubes: Blue (Sodium Citrate) tube 2.7mL
Additional Information: Factor V Leiden is part of Thrombophilia screen.

Thrombophilia testing will be performed for the following clinical indications:

Idiopathic venous thrombo-embolism in young patients (<45 years) Recurrent 
VTE
Recurrent VTE despite adequate therapeutic anticoagulation
VTE in context of family history of unprovoked VTE in a first degree             
relative.
Thrombosis in unusual sites (e.g. cerebral, mesenteric, portal)               VTE 
in association with a history of thrombophlebitis
Warfarin-induced skin necrosis
Stillbirth
Pregnancy complications such as pre-eclampsia, IUGR, implantation failure,  
placental abruption or recurrent miscarriage.*  

Thrombophilia screen will not be performed in the following clinical settings:

Prior to prescription of COC in patients with a personal history of VTE† 
Prior to prescription of COC in patients with a family history of VTE†
Arterial thrombosis (Lupus testing is indicated in this setting)

*   There is no evidence currently to support anticoagulation in women with 
pregnancy complications and a positive hereditary thrombophilia.  Trials are 
ongoing in this area.  However, it is recognised that in routine practice 
management decisions are currently made in conjunction with thrombophilia testing.

†   Current British guidelines recommend avoidance of the COC in this setting 
regardless of the thrombophilia results.

PLEASE NOTE:  As of 09.01.2012 Activated Protein C Resistance is no longer 
performed as part of a thrombophilia screen.


Referred Specimens:
Minimum Aliquot: 3mL
Pre-Transport Requirements to Reference Lab: Room temperature
Transport Requirements to Reference Lab: Room temperature
In the Lab:
Delphic Code: FVPT
Ultra Code: TMG
Testing Schedule: Fortnightly
Turnaround Time: 14 days
Method: LightCycler - PCR
Stability Time Limit for Add-On Tests: No add on testing possible
Link to Application and Diagnostic Use Website: https://www.rcpa.edu.au/Library/Practising-Pathology/RCPA-Manual/Items/Pathology-Tests
Contact Details:
Lab Contact: Chor Ee Tan
Department Name: Molecular Biology
Phone: (04) 918 5314

Test Information last updated on 11/01/2017
Website last updated on 27/03/2017 08:32

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