|SYPHILIS EIA SCREEN|
|Alternate Names for Test:||
FLUORESCENT TREPONEMAL ANITBODY - Test no longer performed
FTA - test no longer performed
SEROLOGICAL TESTS FOR SYPHILIS
STANDARD TEST FOR SYPHILIS
VENERAL DISEASE RESEARCH LABORATORY
|Constituent Tests:||Syphilis EIA screen. If Positive then confirmatory RPR, TPPA|
|Specimen Collection Requirements:|
1x Yellow SST (Serum Separator) tube 5.0mL
|Minimum Sample Volume:||0.6 mL serum|
|Alternative Blood Tubes:||Mauve (EDTA) tube 4.0mL|
|In the Lab:|
|Lab Solutions Code:||SYPE|
|Testing Schedule:||Analysed daily, Monday - Friday.|
|Turnaround Time:||Daily, Monday - Friday|
|Reference Interval & Interpretation:||Non Reactive
Further results to follow
|Method:||Abbott Architect Syphilis TP - CMIA|
|Stability Time Limit for Add-On Tests:||5 days|
|Reflex / Confirmatory Tests:||RPR & TPPA|
|Link to Application and Diagnostic Use Website:||http://www.rcpamanual.edu.au/index.php?option=com_pttests&view=tests&Itemid=77|
Positive serum aliquots stored in freezer (for approx 1 - 2 years) for any
testing that may be required.
SYPHILIS EIA Screen
No diagnostic test provides absolute assurance that a sample does not contain
low levels of antibodies to TP,
such as those present at a very early stage of infection.Therefore, a negative
result at any time does not preclude
the possibility of exposure to infection with syphilis.
Since reactivity in non-treponemal tests for syphilis is a result of tissue
damage, false negative results may occur
in stages of the disease where tissue damage is minimal.
This is particularly true in early primary infections and during latent stages.
Biological False Positives have been reported in patients with autoimmune
disease (SLE, AntiPhospholipid syndrome),
viral infections, malaria, liprosy, yaws and a wide variety of other
conditions including pregnancy.
At the early stage of infection asay may not be sensitive enough to detect
specific antibody. Therefore when
infection is suspected, repeat sampling (2-3 weeks later) should be performed
and interpreted in conjunction with
clinical symptoms, clinical history and other available data.
patients with suspected syphilis and contacts;
blood and tissue donors; patients with STD, HIV infection.
EIA are used as screening tests.
TPPA are used as confirmatory tests.
Serum tests: the RPR and VDRL are sensitive but non-specific
tests. Positive results may indicate active syphilis but
confirmatory tests for specific antibody to T. pallidum are
required. RPR or VDRL are also used for monitoring treatment.
The titre falls with successful treatment, but these tests
may not become negative unless treatment is commenced early in
the course of the infection.
Biological false positives may be found in pregnancy;
transiently in eg, measles, chicken pox; chronically in eg,
cirrhosis, SLE, the phospholipid antibody syndrome, leprosy.
TPPA: positive results confirm the diagnosis of syphilis, but
do not indicate whether the disease is active, inactive or
Titres may remain elevated after effective therapy, although
they may become negative if treatment has been commenced early.
Egglestone SI and Turner AJL Commun Dis Public Health 2000; 3: 158-162.
|Lab Contact:||Paul Tustin & Glennis White|
Test Information last updated on 05/11/2013
Website last updated on 21/11/2015 11:46