|SYPHILIS EIA SCREEN|
|Alternate Names for Test:||
SEROLOGICAL TESTS FOR SYPHILIS
STANDARD TEST FOR SYPHILIS
VENERAL DISEASE RESEARCH LABORATORY
|Constituent Tests:||Syphilis EIA screen. If Positive then confirmatory RPR, TPPA|
|Specimen Collection Requirements:|
1x Yellow SST (Serum Separator) tube 8.5mL
|Minimum Sample Volume:||0.6 mL serum|
|Alternative Blood Tubes:||Mauve (EDTA) tube 4.0mL|
|In the Lab:|
|Lab Solutions Code:||SYPE|
|Testing Schedule:||Analysed daily, Monday - Friday.|
|Turnaround Time:||Daily, Monday - Friday|
|Reference Interval & Interpretation:||Non Reactive
Further results to follow
|Method:||Abbott Architect Syphilis TP - CMIA|
|Stability Time Limit for Add-On Tests:||5 days|
|Reflex / Confirmatory Tests:||RPR & TPPA|
|Link to Application and Diagnostic Use Website:||https://www.rcpa.edu.au/Library/Practising-Pathology/RCPA-Manual/Items/Pathology-Tests|
Positive serum aliquots stored in freezer (for approx 1 - 2 years) for any future
testing that may be required.
SYPHILIS EIA Screen
No diagnostic test provides absolute assurance that a sample does not contain low
levels of antibodies to TP, such as those present at a very early stage of
infection. Therefore, a negative result at any time does not preclude
the possibility of exposure to infection with syphilis.
Since reactivity in non-treponemal tests for syphilis is a result of tissue damage,
false negative results may occur in stages of the disease where tissue damage is
minimal. This is particularly true in early primary infections and during latent
stages. Biological False Positives have been reported in patients with autoimmune
disease (SLE, AntiPhospholipid syndrome), viral infections, malaria, leprosy, yaws
and a wide variety of other conditions including pregnancy.
At the early stage of infection asay may not be sensitive enough to detect
specific antibody. Therefore when infection is suspected, repeat sampling (2-3
weeks later) should be performed and interpreted in conjunction with clinical
symptoms, clinical history and other available data.
Serum tests: patients with suspected syphilis and contacts; antenatal screening;
blood and tissue donors; patients with STD, HIV infection.
EIA are used as screening tests.
TPPA are used as confirmatory tests.
Serum tests: the RPR and VDRL are sensitive but non-specific tests. Positive
results may indicate active syphilis but confirmatory tests for specific antibody
to T. pallidum are required. RPR or VDRL are also used for monitoring treatment.
The titre falls with successful treatment, but these tests may not become negative
unless treatment is commenced early in the course of the infection. Biological
false positives may be found in pregnancy; transiently in eg, measles, chicken pox;
chronically in eg, cirrhosis, SLE, the phospholipid antibody syndrome, leprosy.
TPPA: positive results confirm the diagnosis of syphilis, but do not indicate
whether the disease is active, inactive or cured. Titres may remain elevated after
effective therapy, although they may become negative if treatment has been
Reference: Egglestone SI and Turner AJL Commun Dis Public Health 2000; 3: 158-162.
|Lab Contact:||Paul Tustin|
Test Information last updated on 12/06/2016
Website last updated on 27/06/2016 08:19